CRISPR/Cas9 Gene Targeting in Fertilized Eggs (Bold Fields Required)
PI Last Name:
Account Number:
IACUC Protocol Number:
Gene/Mutation/Project Name:
Nuclease Type: (default is Cas9 nuclease)	ZFNTALENCas9 NucleaseCas9 Nickase
Mutation Type:	small indelssmall editinsertiondeletion
Type of Targeting Vector:	nonessDNA oligonucleotidesupercoiled circular plasmid
Size of Targeting Vector:
Mouse Strain to Inject:	B6SJLFVB + $1500 C57BL/6J + $1500Other + $1500 or more     If other, describe below.
Are null homozygotes sick/infertile/dead?	YesNo     If yes, explain below.
Other Information:
Building and Room to Deliver Mice to:
NIH Recombinant DNA Regulatory Information
If you have an approved CWRU IBC protocol number enter it here.
If you have an approved CWRU IBC protocol number for this work, enter it. If you do not, answer all the following questions.
Describe the scientific purpose and design of these experiments.
Describe the goals in a way which would be comprehensible to the public.
Is the gene or mutation associated with infectious disease in healthy adult humans?	YesNo
Are the hazards to personnel and the environment minimal or absent?	YesNo
Which gene is targeted gene in mice (e.g. beta-actin)
Describe the function of the gene targeted in mice (e.g. cytoskeleton)
Upload a pdf diagram of the targeting strategy