Gene Targeting Request (Bold Fields required)
PI Last Name:
Account:
What is the BioSafety Level?
BSL1
BSL2
BSL3
(Most work is BSL1)
Which Cell Line to Target?
R1 (129) $5500
C2 (C57BL/6) $7700
PC3 (129/PrmCre) $7700
Other $7700
Targeting Construct Name:
DNA Concentration (µg/µl):
DNA Amount (µg):
Targeting Construct Total Size (kb):
Length of 5 prime arm (kb):
Length of 3 prime arm (kb):
Enzyme(s) to linearize construct:
Positive Selectable Marker
Neo
Puro
Hygro
Hprt
beta-Geo
Other
Negative Selectable Marker:
tk
dta
Other
None
Southern Blot Will Be Provided:
Yes
No
LoxP's verified by sequence or activity
Yes
No
N/A
Special Considerations:
(loxP sites verified by sequence or activity, conditional mutant, knockin, point mutant, floxed neo, special cell line, etc.)