We create transgenic mice, knockout mice (null mutations, knockins, targeted point mutations, humanized genes, conditional knockouts) and chimeric mice, provide rederivation, cryopreservation services and establish embryonic stem cell lines for the Cleveland Biomedical Research Community.
The Case Transgenic and Targeting Facility office is located in Wolstein Research Bldg 2521.
Services are contracted on a fee-for-service, first-come-first-serve basis. Our rates are low, our wait times are short, and we have an oustanding record of success.
| Weihong Jiang | Staff | 368-2528 | wxj13 |
| David LePage | Staff | 368-2528 | dfl |
| Rachel Mann | Manager | 368-2528 | rjm12 |
| Ron Conlon | Director | 368-1826 | rac14 |
The Case Rodent Behavior Core offers discounts on their services for mice generated by the Transgenic and Targeting Facility.
- Transgenics
- Targeting
- Chimeras
- Rederivation
- Cryopreservation
- Generation of new ES cell lines
- ES cell transfection
- ES cell homozygosis
- In vitro Fertilization (IVF)
- Intracytoplasmic Sperm Injection (ICSI)
- Frozen Embryo Transfer (FET)
- Ovariectomy, Vasectomy and Other Surgeries
- Cost of Services
- Turnaround Times
- NIH Supplements for making mice from genetrap cell lines
- Mice: The Basics
- How to Breed Mice
- How to Design Gene Targeting Vectors
- Existing Mutants and Where to Get Them
- Commercial DNA Cloning and Genotyping Services
- What an Incoming Investigator Needs to Know
- Health status of all CWRU mice 06/08 (.pdf)
- Protocols
- Resources and Links
It is the investigator's responsibility to ensure that they are in compliance with the regulations of the Institutional Biosafety Committee (IBC) and the Institutional Animal Care and Use Committee (IACUC). Investigators must have an approved IBC protocol from the CWRU IBC for the creation of transgenic and chimeric mice. Investigators must have an approved IACUC protocol from their institution's IACUC to receive animals from our service.
| Service | Time to Job Start* (8/28/08) |
| Gene Targeting | 8 weeks |
| Transgenics | 3 weeks |
| Chimeras | 3 weeks |
| Rederivation | 3 weeks |
| Cryopreservation | immediately |
| *after receipt and validation of materials and information | |
Left to Right: Ron, Rachel, David, Weihong