SOM     CCCC     CTSC
    We are leaders in CRISPR/Cas9 gene targeting. We have completed over 40 CRISPR/Cas9 projects including knockouts, reporter and point mutant knockins and targeted transgenics. more details.

     Our fees are low, our wait times are short and our success rates are high. New transgenic and targeted mice that we have created are featured in over 200 publications since 2004.

    We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center and the Clinical and Translational Science Collaborative.

NameRoleOfficePhone
Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 623 368-1826


ctsc
Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.
Transgenics Targeting Chimeras Rederivation CRISPR/Cas Cryo IVF FET Other

Transgenic and Targeted Mice Made by the Core

2018
  1. Cheng-Hathaway PJ, Reed-Geaghan EG, Jay TR, Casali BT, Bemiller SM, Puntambekar SS, von Saucken VE, Williams RY, Karlo JC, Moutinho M, Xu G, Ransohoff RM, Lamb BT, Landreth GE. The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease. Mol Neurodegener. 2018 13(1):29 PMID: 29859094
    • A disease-variant allele made by CRISPR/Cas9 mutagenesis.

  2. Meier ID, Walker MP, Matera AG. Gemin4 is an essential gene in mice, and its overexpression in human cells causes relocalization of the SMN complex to the nucleoplasm. Biol Open. 2018 PMID: 29371219

2017
  1. Orru CD, Yuan J, Appleby BS, Li B, Li Y, Winner D, Wang Z, Zhan YA, Rodgers M, Rarick J, Wyza RE, Joshi T, Wang GX, Cohen ML, Zhang S, Groveman BR, Petersen RB, Ironside JW, Quiñones-Mateu ME, Safar JG, Kong Q, Caughey B, Zou WQ. Prion seeding activity and infectivity in skin samples from patients with sporadic Creutzfeldt-Jakob disease. Sci Transl Med. 2017 9(417). PMID: 29167394

  2. Matarazzo V, Caccialupi L, Schaller F, Shvarev Y, Kourdougli N, Bertoni A, Menuet C, Voituron N, Deneris E, Gaspar P, Bezin L, Durbec P, Hilaire G, Muscatelli F. Necdin shapes serotonergic development and SERT activity modulating breathing in a mouse model for Prader-Willi Syndrome. Elife. 2017 PMID: 29087295

  3. Geng R, Omar A, Gopal SR, Chen DH, Stepanyan R, Basch ML, Dinculescu A, Furness DN, Saperstein D, Hauswirth W, Lustig LR, Alagramam KN. Modeling and Preventing Progressive Hearing Loss in Usher Syndrome III. Sci Rep. 2017 7(1):13480. PMID: 29044151

  4. Vithayathil J, Pucilowska J, Friel D, Landreth GE. Chronic impairment of ERK signaling in glutamatergic neurons of the forebrain does not affect spatial memory retention and LTP in the same manner as acute blockade of the ERK pathway. Hippocampus. 2017 PMID: 28833860

  5. Wichman L, Somasundaram S, Breindel C, Valerio DM, McCarrey JR, Hodges CA, Khalil AM. Dynamic expression of long non-coding RNAs reveals their potential roles in spermatogenesis and fertility. Biol Reprod 2017
    • This publication describes an lncRNA gene deletion generated with CRISPR/Cas9 by the core

  6. Hutton SR, Otis JM, Kim EM, Lamsal Y, Stuber GD, Snider WD. ERK/MAPK signaling is required for pathway-specific striatal motor functions. J Neurosci. 2017

  7. Soler DC, Young AE, Griffith AD, Fu PF, Cooper KD, McCormick TS, Popkin DL. Overexpression of AQP3 and AQP10 in the skin exacerbates psoriasiform acanthosis. Exp Dermatol. 2017 PMID: 28111811

  8. Seachrist DD, Sizemore ST, Johnson E, Abdul-Karim FW, Weber Bonk KL, Keri RA. Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer. Breast Cancer Res. 2017 PMID: 28583174

  9. Nguyen HT, Hinman MN, Guo X, Sharma A, Arakawa H, Luo G, Lou H. Neurofibromatosis type 1 alternative splicing is a key regulator of Ras/ERK signaling and learning behaviors in mice. Hum Mol Genet 2017

  10. Wilk K, Yeh SA, Mortensen LJ, Ghaffarigarakani S, Lombardo CM, Bassir SH, Aldawood ZA, Lin CP, Intini G. Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration. Stem Cell Reports. 2017 PMID: 28366454

  11. Kurtsdotter I, Topcic D, Karlén A, Singla B, Hagey DW, Bergsland M, Siesjö P, Nistér M, Carlson JW, Lefebvre V, Persson O, Holmberg J, Muhr J. SOX5/6/21 prevent oncogene-driven transformation of brain stem cells. Cancer Res. 2017 PMID: 28687615

  12. Schugar RC, Shih DM, Warrier M, Helsley RN, Burrows A, Ferguson D, Brown AL, Gromovsky AD, Heine M, Chatterjee A, Li L, Li XS, Wang Z, Willard B, Meng Y, Kim H, Che N, Pan C, Lee RG, Crooke RM, Graham MJ, Morton RE, Langefeld CD, Das SK, Rudel LL, Zein N, McCullough AJ, Dasarathy S, Tang WHW, Erokwu BO, Flask CA, Laakso M, Civelek M, Naga Prasad SV, Heeren J, Lusis AJ, Hazen SL, Brown JM. The TMAO-Producing Enzyme Flavin-Containing Monooxygenase 3 Regulates Obesity and the Beiging of White Adipose Tissue. Cell Rep. 2017 19(12):2451-2461. PMID: 28636934
    • This publication describes Fmo3 knockout mice generated with CRISPR/Cas9 by the core

  13. Khoriaty R, Vogel N, Hoenerhoff MJ, Sans MD, Zhu G, Everett L, Nelson B, Durairaj H, McKnight B, Zhang B, Ernst SA, Ginsburg D, Williams JA. SEC23B is required for pancreatic acinar cell function in adult mice. Mol Biol Cell. 2017 PMID: 28539403

  14. Darrah R, Bederman I, Vitko M, Valerio DM, Drumm ML, Hodges CA. Growth deficits in cystic fibrosis mice begin in utero prior to IGF-1 reduction. PLoS One. 2017 12(4):e0175467. PMID: 28384265

  15. Mamidi R, Gresham KS, Li J, Stelzer JE. Cardiac myosin binding protein-C Ser302 phosphorylation regulates cardiac β-adrenergic reserve. Sci Adv. 2017 3(3):e1602445. PMID: 28345052

  16. Kuwajima T, Soares CA, Sitko AA, Lefebvre V, Mason C. SoxC Transcription Factors Promote Contralateral Retinal Ganglion Cell Differentiation and Axon Guidance in the Mouse Visual System. Neuron. 2017 PMID: 28215559

2016
  1. Jacobsen CM, Schwartz MA, Roberts HJ, Lim KE, Spevak L, Boskey AL, Zurakowski D, Robling AG, Warman ML. Enhanced Wnt signaling improves bone mass and strength, but not brittleness, in the Col1a1(+/mov13) mouse model of type I Osteogenesis Imperfecta. Bone. 2016 90:127-32 PMID: 27297606

  2. Lanza DG, Dawson EP, Rao P, Heaney JD. Misexpression of cyclin D1 in embryonic germ cells promotes testicular teratoma initiation. Cell Cycle. 2016;15(7):919-30. PMID: 26901436

  3. Fritz Y, Klenotic PA, Swindell WR, Yin Z, Groft SG, Zhang L, Baliwag J, Camhi MI, Diaconu D, Young AB, Foster AM, Johnston A, Gudjonsson JE, McCormick TS, Ward NL. Induction of alternative proinflammatory cytokines accounts for sustained psoriasiform skin inflammation in IL-17C+IL-6KO mice. J Invest Dermatol. 2016 PMID: 27984037

  4. Jay TR, Hirsch AM, Broihier ML, Miller CM, Neilson LE, Ransohoff RM, Lamb BT, Landreth GE. Disease progression-dependent effects of TREM2 deficiency in a mouse model of Alzheimer's disease. J Neurosci. 2016 PMID: 27940990

  5. Jiang K, Jiao S, Vitko M, Darrah R, Flask CA, Hodges CA, Yu X. The impact of Cystic Fibrosis Transmembrane Regulator Disruption on cardiac function and stress response. J Cyst Fibros. 2016 Jan;15(1):34-42. PMID: 26119592

  6. Charrier A, Wang L, Stephenson EJ, Ghanta SV, Ko CW, Croniger CM, Bridges D, Buchner DA. Zinc finger protein 407 overexpression upregulates PPAR-target gene expression and improves glucose homeostasis in mice. Am J Physiol Endocrinol Metab. 2016 PMID: 27624101

  7. Portillo JC, Corcino YL, Miao Y, Tang J, Sheibani N, Kern TS, Dubyak GR, Subauste CS. CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes. 2016 PMID: 27474370

  8. Ghosal K, Fan Q, Dawson HN, Pimplikar SW. Tau Protein Mediates APP Intracellular Domain (AICD)-Induced Alzheimer's-Like Pathological Features in Mice. PLoS One. 2016 11(7):e0159435. PMID: 27459671

  9. Khoriaty R, Everett L, Chase J, Zhu G, Hoenerhoff M, McKnight B, Vasievich MP, Zhang B, Tomberg K, Williams J, Maillard I, Ginsburg D. Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice. Sci Rep. 2016 6:27802. PMID: 27297878

  10. Bomben VC, Aiba I, Qian J, Mark MD, Herlitze S, Noebels JL. Isolated P/Q Calcium Channel Deletion in Layer VI Corticothalamic Neurons Generates Absence Epilepsy. J Neurosci. 2016 36(2):405-18. PMID: 26758833

  11. Tilot AK, Bebek G, Niazi F, Altemus JB, Romigh T, Frazier TW, Eng C. Neural transcriptome of constitutional Pten dysfunction in mice and its relevance to human idiopathic autism spectrum disorder. Mol Psychiatry. 2016 21(1):118-25. PMID: 25754085

  12. Alagramam KN, Gopal SR, Geng R, Chen DH, Nemet I, Lee R, Tian G, Miyagi M, Malagu KF, Lock CJ, Esmieu WR, Owens AP, Lindsay NA, Ouwehand K, Albertus F, Fischer DF, Bürli RW, MacLeod AM, Harte WE, Palczewski K, Imanishi Y. A small molecule mitigates hearing loss in a mouse model of Usher syndrome III. Nat Chem Biol. 2016 PMID: 27110679

  13. Bruggeman LA, Wu Z, Luo L, Madhavan SM, Konieczkowski M, Drawz PE, Thomas DB, Barisoni L, Sedor JR, O'Toole JF. APOL1-G0 or APOL1-G2 Transgenic Models Develop Preeclampsia but Not Kidney Disease. J Am Soc Nephrol. 2016 PMID: 27026370

  14. Saber M, Kokiko-Cochran ON, Puntambekar S, Lathia J, Lamb BT. TREM2 deficiency alters acute macrophage distribution and improves recovery after TBI. J Neurotrauma. 2016 PMID: 26976047

  15. Wyler SC, Spencer WC, Green NH, Rood BD, Crawford L, Craige C, Gresch P, McMahon DG, Beck SG, Deneris E. Pet-1 Switches Transcriptional Targets Postnatally to Regulate Maturation of Serotonin Neuron Excitability. J Neurosci. 2016 Feb 3;36(5):1758-74. PMID: 26843655

  16. Than BL, Linnekamp JF, Starr TK, Largaespada DA, Rod A, Zhang Y, Bruner V, Abrahante J, Schumann A, Luczak T, Niemczyk A, O'Sullivan MG, Medema JP, Fijneman RJ, Meijer GA, Van den Broek E, Hodges CA, Scott PM, Vermeulen L, Cormier RT. CFTR is a tumor suppressor gene in murine and human intestinal cancer. Oncogene. 2016 PMID: 26751771

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