We are leaders in transgenics and CRISPR/Cas gene targeting. We have almost 20 different CRISPR knockin/out projects at different stages of completion, seven of which have progressed to the point of successful germline transmission of the desired allele, and two which have generated homozygotes in the second generation with the expected phenotype.

     Our fees are low, our wait times are short and our success rates are high. New transgenic and targeted mice that we have created are featured in over 200 publications since 2004.

    We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center and the Clinical and Translational Science Collaborative.

Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 623 368-1826

Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.

Transgenic and Targeted Mice Made by the Core

  1. Berisha SZ, Brubaker G, Kasumov T, Hung KT, DiBello PM, Huang Y, Li L, Willard B, Pollard KA, Nagy LE, Hazen SL, Smith JD. HDL from apoA1 transgenic mice expressing the 4WF isoform is resistant to oxidative loss of function. J Lipid Res. 2015 Mar;56(3):653-64. PMID: 25561462

  2. Niziolek PJ, Bullock W, Warman ML, Robling AG. Missense Mutations in LRP5 Associated with High Bone Mass Protect the Mouse Skeleton from Disuse- and Ovariectomy-Induced Osteopenia. PLoS One. 2015 10(11):e0140775. PMID: 26554834

  3. Gnedeva K, Hudspeth AJ. SoxC transcription factors are essential for the development of the inner ear. Proc Natl Acad Sci U S A. 2015 112(45): 14066-14071 PMID: 26504244

  4. Baganz NL, Lindler KM, Zhu CB, Smith JT, Robson MJ, Iwamoto H, Deneris ES, Hewlett WA, Blakely RD. A requirement of serotonergic p38α mitogen-activated protein kinase for peripheral immune system activation of CNS serotonin uptake and serotonin-linked behaviors. Transl Psychiatry. 2015 Nov 3;5:e671. PMID: 26529424

  5. Parada C, Han D, Grimaldi A, Sarrión P, Park SS, Pelikan R, Sanchez-Lara PA, Chai Y. Disruption of the ERK/MAPK pathway in neural crest cells as a potential cause of Pierre Robin sequence. Development. 2015 PMID: 26395480

  6. Richardson ET, Shukla S, Nagy N, Boom WH, Beck RC, Zhou L, Landreth GE, Harding CV. ERK Signaling Is Essential for Macrophage Development. PLoS One. 2015 10(10):e0140064. PMID: 26445168

  7. Zhao J, Bulek K, Gulen MF, Zepp JA, Karagkounis G, Martin BN, Zhou H, Yu M, Liu X, Huang E, Fox PL, Kalady MF, Markowitz SD, Li X. Human Colon Tumors Express a Dominant-negative Form of SIGIRR That Promotes Inflammation and Colitis-associated Colon Cancer in Mice. Gastroenterology. 2015 PMID: 26344057

  8. Poncy A, Antoniou A, Cordi S, Pierreux CE, Jacquemin P, Lemaigre FP. Transcription factors SOX4 and SOX9 cooperatively control development of bile ducts. Dev Biol. 2015 PMID: 26033091

  9. Liu CF, Lefebvre V. The transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide through super-enhancers to drive chondrogenesis. Nucleic Acids Res. 2015 PMID: 26150426

  10. Huang AH, Riordan TJ, Pryce B, Weibel JL, Watson SS, Long F, Lefebvre V, Harfe BD, Stadler HS, Akiyama H, Tufa SF, Keene DR, Schweitzer R. Musculoskeletal integration at the wrist underlies modular development of limb tendons. Development. 2015 PMID: 26062940

  11. O'Brien DE, Alter BJ, Satomoto M, Morgan CD, Davidson S, Vogt SK, Norman ME, Gereau GB, Demaro JA 3rd, Landreth GE, Golden JP, Gereau RW 4th. ERK2 Alone Drives Inflammatory Pain But Cooperates with ERK1 in Sensory Neuron Survival. J Neurosci. 2015 35(25):9491-507 PMID: 26109671

  12. Mark MD, Krause M, Boele HJ, Kruse W, Pollok S, Kuner T, Dalkara D, Koekkoek S, De Zeeuw CI, Herlitze S. Spinocerebellar Ataxia Type 6 Protein Aggregates Cause Deficits in Motor Learning and Cerebellar Plasticity. J Neurosci. 2015 35(23):8882-95 PMID: 26063920

  13. Nandadasa S, Nelson CM, Apte SS. ADAMTS9-Mediated Extracellular Matrix Dynamics Regulates Umbilical Cord Vascular Smooth Muscle Differentiation and Rotation. Cell Rep. 2015 PMID: 26027930

  14. Margevicius DR, Bastian C, Fan Q, Davis RJ, Pimplikar SW. JNK-interacting protein 1 mediates Alzheimer's-like pathological features in AICD-transgenic mice. Neurobiol Aging. 2015 PMID: 26022769

  15. Yao B, Wang Q, Liu CF, Bhattaram P, Li W, Mead TJ, Crish JF, Lefebvre V. The SOX9 upstream region prone to chromosomal aberrations causing campomelic dysplasia contains multiple cartilage enhancers. Nucleic Acids Res. 2015 PMID: 25940622

  16. Vithayathil J, Pucilowska J, Goodnough LH, Atit RP, Landreth GE. Dentate Gyrus Development Requires ERK Activity to Maintain Progenitor Population and MAPK Pathway Feedback Regulation. J Neurosci. 2015 35(17):6836-48 PMID: 25926459

  17. McGlashon, Gorecki, Kozlowski, Thimbeck, Markan, Lesli, Kotas, Potthoff, Richerson and Gillum. Central serotonergic neurons activate and recruit thermogenic brown and beige fat and regulate glucose and lipid homeostasis. 2015, Cell Metabolism 21, 692–705

  18. Kato K, Bhattaram P, Penzo-Méndez A, Gadi A, Lefebvre V. SOXC Transcription Factors Induce Cartilage Growth Plate Formation in Mouse Embryos by Promoting Noncanonical WNT Signaling. J Bone Miner Res. 2015 PMID: 25761772

  19. Niziolek PJ, MacDonald BT, Kedlaya R, Zhang M, Bellido T, He X, Warman ML, Robling AG. High-bone-mass causing mutant LRP5 receptors are resistant to endogenous inhibitors in vivo. J Bone Miner Res. 2015 PMID: 25808845

  20. Jay TR, Miller CM, Cheng PJ, Graham LC, Bemiller S, Broihier ML, Xu G, Margevicius D, Karlo JC, Sousa GL, Cotleur AC, Butovsky O, Bekris L, Staugaitis SM, Leverenz JB, Pimplikar SW, Landreth GE, Howell GR, Ransohoff RM, Lamb BT. TREM2 deficiency eliminates TREM2+ inflammatory macrophages and ameliorates pathology in Alzheimer's disease mouse models. J Exp Med. 2015 PMID: 25732305

  21. Bhattaram P, Penzo-Méndez A, Kato K, Bandyopadhyay K, Gadi A, Taketo MM, Lefebvre V. SOXC proteins amplify canonical WNT signaling to secure nonchondrocytic fates in skeletogenesis. J Cell Biol. 2014 207(5):657-71. PMID: 25452386

  22. Berger M, Scheel DW, Macias H, Miyatsuka T, Kim H, Hoang P, Ku GM, Honig G, Liou A, Tang Y, Regard JB, Sharifnia P, Yu L, Wang J, Coughlin SR, Conklin BR, Deneris ES, Tecott LH, German MS. Gαi/o-coupled receptor signaling restricts pancreatic β-cell expansion. Proc Natl Acad Sci U S A. 2015 PMID: 25695968

  23. Wyler SC, Donovan LJ, Yeager M, Deneris E. Pet-1 controls tetrahydrobiopterin pathway and Slc22a3 transporter genes in serotonin neurons. ACS Chem Neurosci. 2015 PMID: 25642596

  24. Seaberg B, Henslee G, Wang S, Paez-Colasante X, Landreth GE, Rimer M. Muscle-derived ERK1/2 MAP kinases are required for the maintenance of adult myofibers and their neuromuscular junctions. Mol Cell Biol. 2015 PMID: 25605336

  25. Berisha SZ, Brubaker G, Kasumov T, Hung KT, DiBello PM, Huang Y, Li L, Willard B, Pollard KA, Nagy LE, Hazen SL, Smith JD. HDL from ApoA1 Transgenic Mice Expressing the 4WF Isoform Is Resistant to Oxidative Loss of Function. J Lipid Res. 2015 PMID: 25561462

  26. Afelik S, Pool B, Schmerr M, Penton C, Jensen J. Wnt7b is required for epithelial progenitor growth and operates during epithelial-to-mesenchymal signaling in pancreatic development. Dev Biol. 2015 PMID: 25576928

  1. Tilot AK, Gaugler MK, Yu Q, Romigh T, Yu W, Miller RH, Frazier TW 2nd, Eng C. Germline disruption of Pten localization causes enhanced sex-dependent social motivation and increased glial production. Hum Mol Genet. 2014 23(12):3212-27. PMID: 24470394

  2. Cerpa V, Gonzalez A, Richerson GB Diphtheria toxin treatment of Pet-1-Cre floxed diphtheria toxin receptor mice disrupts thermoregulation without affecting respiratory chemoreception. Neuroscience. 2014 279:65-76.

  3. Chen Z, Yue SX, Zhou G, Greenfield EM, Murakami S. ERK1 and ERK2 regulate chondrocyte terminal differentiation during endochondral bone formation. J Bone Miner Res. 2014 PMID: 25401279

  4. Ng HP, Zhou Y, Song K, Hodges CA, Drumm ML, Wang G. Neutrophil-mediated phagocytic host defense defect in myeloid cftr-inactivated mice. PLoS One. 2014 9(9):e106813. PMID: 25184794

  5. Khoriaty R, Vasievich MP, Jones M, Everett L, Chase J, Tao J, Siemieniak D, Zhang B, Maillard I, Ginsburg D. Absence of a red blood cell phenotype in mice with hematopoietic deficiency of SEC23B. Mol Cell Biol. 2014 PMID: 25071156

  6. Yin X, Kiryu-Seo S, Kidd GJ, Feltri ML, Wrabetz L, Trapp BD. Proteolipid protein cannot replace P0 protein as the major structural protein of peripheral nervous system myelin. Glia. 2014 PMID: 25066805

  7. Issler O, Haramati S, Paul ED, Maeno H, Navon I, Zwang R, Gil S, Mayberg HS, Dunlop BW, Menke A, Awatramani R, Binder EB, Deneris ES, Lowry CA, Chen A. MicroRNA 135 Is Essential for Chronic Stress Resiliency, Antidepressant Efficacy, and Intact Serotonergic Activity. 2014 Neuron. 6273(14)00486-3. PMID: 24952960

  8. Gresham KS, Mamidi R, Stelzer JE. The contribution of cMyBP-C Ser282 phosphorylation to the rate of force generation and in vivo cardiac contractility. J Physiol. 2014 PMID: 24951619

  9. Hinman MN, Sharma A, Luo G, Lou H. Neurofibromatosis Type 1 Alternative Splicing Is a Key Regulator of Ras Signaling in Neurons. Mol Cell Biol. 2014 34(12):2188-2197. PMID: 24710274

  10. Arachiche A, de la Fuente M, Nieman MT. Platelet Specific Promoters Are Insufficient to Express Protease Activated Receptor 1 (PAR1) Transgene in Mouse Platelets. PLoS One. 2014 9(5):e97724. PMID: 24830314

  11. Spoida K, Masseck OA, Deneris ES, Herlitze S. Gq/5-HT2c receptor signals activate a local GABAergic inhibitory feedback circuit to modulate serotonergic firing and anxiety in mice. Proc Natl Acad Sci U S A. 2014 PMID: 24733892

  12. Jacobsen CM, Barber LA, Ayturk UM, Roberts HJ, Deal LE, Schwartz MA, Weis M, Eyre D, Zurakowski D, Robling AG, Warman ML. Targeting the LRP5 Pathway Improves Bone Properties in a Mouse Model of Osteogenesis Imperfecta. J Bone Miner Res. 2014 PMID: 24677211