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    We are leaders in BAC transgenics and gene targeting.

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    The Case Transgenic and Targeting Core Facility fees are low, our wait times are short and we have outstanding success rates with over 150 publications of transgenic and targeted mice resulting from our work.

    We are supported by the Case Western Reserve University School of Medicine and the Case Comprehensive Cancer Center.

NameRoleOfficePhone
Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 623 368-1826


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Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.
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Transgenic and Targeted Mice Made by the Core

2013
  1. Ayturk UM, Jacobsen CM, Christodoulou DC, Gorham J, Seidman JG, Seidman CE, Robling AG, Warman ML. An RNA-seq protocol to identify mRNA expression changes in mouse diaphyseal bone: applications in mice with bone property altering Lrp5 mutations. J Bone Miner Res. 2013 28(10):2081-93. PMID: 23553928

  2. Waller KA, Zhang LX, Elsaid KA, Fleming BC, Warman ML, Jay GD. Role of lubricin and boundary lubrication in the prevention of chondrocyte apoptosis. Proc Natl Acad Sci U S A. 2013 110(15):5852-7. PMID: 23530215

  3. Shi Q, Prior M, Zhou X, Tang X, He W, Hu X, Yan R. Preventing formation of reticulon 3 immunoreactive dystrophic neurites improves cognitive function in mice. J Neurosci. 2013 33(7):3059-66. PMID: 23407961

  4. Kedlaya R, Veera S, Horan DJ, Moss RE, Ayturk UM, Jacobsen CM, Bowen ME, Paszty C, Warman ML, Robling AG. Sclerostin Inhibition Reverses Skeletal Fragility in an Lrp5-Deficient Mouse Model of OPPG Syndrome. Sci Transl Med. 2013 5(211):211ra158. PMID: 24225945

  5. Luo X, He W, Hu X, Yan R. Reversible Overexpression of Bace1-Cleaved Neuregulin-1 N-Terminal Fragment Induces Schizophrenia-Like Phenotypes in Mice. Biol Psychiatry. 2013 PMID: 24210810

  6. Yeh IJ, Ogba N, Bensigner H, Welford SM, Montano MM. HEXIM1 down-regulates hypoxia-inducible factor-1α protein stability. Biochem J. 2013 456(2):195-204 PMID: 24015760

  7. Zhang H, Alberich-Jorda M, Amabile G, Yang H, Staber PB, Diruscio A, Welner RS, Ebralidze A, Zhang J, Levantini E, Lefebvre V, Valk PJ, Delwel R, Hoogenkamp M, Nerlov C, Cammenga J, Saez B, Scadden DT, Bonifer C, Ye M, Tenen DG. Sox4 Is a Key Oncogenic Target in C/EBPα Mutant Acute Myeloid Leukemia. Cancer Cell. 2013 PMID: 24183681

  8. Bükülmez H, Khan F, Bartels CF, Murakami S, Ortiz-Lopez A, Sattar A, Haqqi TM, Warman ML. Protective effects of C-type natriuretic peptide on linear growth and articular cartilage integrity in an inflammatory arthritis mouse model. Arthritis Rheum. 2013 PMID: 24114569

  9. Yuan J, Zhan YA, Abskharon R, Xiao X, Martinez MC, Zhou X, Kneale G, Mikol J, Lehmann S, Surewicz WK, Castilla J, Steyaert J, Zhang S, Kong Q, Petersen RB, Wohlkonig A, Zou WQ. Recombinant Human Prion Protein Inhibits Prion Propagation in vitro. Sci Rep. 2013 Oct 9;3:2911. PMID: 24105336

  10. Ninkovic J, Steiner-Mezzadri A, Jawerka M, Akinci U, Masserdotti G, Petricca S, Fischer J, von Holst A, Beckers J, Lie CD, Petrik D, Miller E, Tang J, Wu J, Lefebvre V, Demmers J, Eisch A, Metzger D, Crabtree G, Irmler M, Poot R, Götz M. The BAF Complex Interacts with Pax6 in Adult Neural Progenitors to Establish a Neurogenic Cross-Regulatory Transcriptional Network. Cell Stem Cell. 2013 PMID: 23933087

  11. Ohta S, Misawa A, Lefebvre V, Okano H, Kawakami Y, Toda M. Sox6 up-regulation by macrophage migration inhibitory factor promotes survival and maintenance of mouse neural stem/progenitor cells. PLoS One. 2013 Sep 16;8(9):e74315. PMID: 24066135

  12. Liu L, Li M, Spangler LC, Spear C, Veenstra M, Darnall L, Chang C, Cotleur AC, Ransohoff RM. Functional defect of peripheral neutrophils in mice with induced deletion of CXCR2. Genesis. 2013 PMID: 23650205

  13. DePuy SD, Stornetta RL, Bochorishvili G, Deisseroth K, Witten I, Coates M, Guyenet PG. Glutamatergic neurotransmission between the C1 neurons and the parasympathetic preganglionic neurons of the dorsal motor nucleus of the vagus. J Neurosci. 2013 33(4):1486-97. PMID: 23345223

  14. Zechel JL, Doerner SK, Lager A, Tesar PJ, Heaney JD, Nadeau JH. Contrasting effects of Deadend1 (Dnd1) gain and loss of function mutations on allelic inheritance, testicular cancer, and intestinal polyposis. BMC Genet. 2013 14:54. PMID: 23773267

  15. He X, Arrotta N, Radhakrishnan D, Wang Y, Romigh T, Eng C. Cowden Syndrome-Related Mutations in PTEN Associate with Enhanced Proteasome Activity. Cancer Res. 2013 73(10):3029-40 PMID: 23475934

  16. Malhotra N, Narayan K, Cho OH, Sylvia KE, Yin C, Melichar H, Rashighi M, Lefebvre V, Harris JE, Berg LJ, Kang J; Immunological Genome Project Consortium. 2013 A network of high-mobility group box transcription factors programs innate interleukin-17 production. Immunity. 38(4):681-93. PMID: 23562159

  17. Jiang Y, Ding Q, Xie X, Libby R, Lefebvre V, Gan L. 2013 Transcription factors SOX4 and SOX11 function redundantly to regulate the development of mouse retinal ganglion cells. J Biol Chem

  18. Montano MM, Desjardins C, Doughman YQ, Hsieh YH, Hu Y, Bensinger H, Wang C, Stelzer J, Dick T, Hoit B, Chandler MP, Yu X, Watanabe M. (2013) Inducible reexpression of HEXIM1 causes physiological cardiac hypertrophy in the adult mouse. Cardiovasc Res. PMID: 23585471

  19. Kiyasova V, Bonnavion P, Scotto-Lomassese S, Fabre V, Sahly I, Tronche F, Deneris E, Gaspar P, Fernandez SP. A subpopulation of serotonergic neurons that do not express the 5-HT1A autoreceptor. ACS Chem Neurosci. 2013 4(1):89-95 PMID: 23336048

  20. Narboux-Nême N, Angenard G, Mosienko V, Klempin F, Pitychoutis PM, Deneris E, Bader M, Giros B, Alenina N, Gaspar P. Postnatal growth defects in mice with constitutive depletion of central serotonin. ACS Chem Neurosci. 2013 4(1):171-81 PMID: 23336056

  21. Sun B, Mallampati S, Gong Y, Wang D, Lefebvre V, Sun X. Sox4 is required for the survival of pro-B cells. J Immunol. 2013 190(5):2080-9. 2013 PMID: 23345330

  22. Mead TJ, Wang Q, Bhattaram P, Dy P, Afelik S, Jensen J, Lefebvre V. A far-upstream (-70 kb) enhancer mediates Sox9 auto-regulation in somatic tissues during development and adult regeneration. Nucleic Acids Res. 2013 PMID: 23449223

2012
  1. Zhou G, Hamik A, Nayak L, Tian H, Shi H, Lu Y, Sharma N, Liao X, Hale A, Boerboom L, Feaver RE, Gao H, Desai A, Schmaier A, Gerson SL, Wang Y, Atkins GB, Blackman BR, Simon DI, Jain MK. Endothelial Kruppel-like factor 4 protects against atherothrombosis in mice. J Clin Invest. 2012 122(12):4727-31. PMID: 23160196

  2. Ramezani-Rad P, Geng H, Hurtz C, Chan LN, Chen Z, Jumaa H, Melnick A, Paietta E, Carroll WL, Willman CL, Lefebvre V, Müschen M. SOX4 enables oncogenic survival signals in acute lymphoblastic leukemia. Blood. 2012 PMID: 23152540

  3. Zhou W, Chen L, Paul J, Yang S, Li F, Sampson K, Woodgett JR, Beaulieu JM, Gamble KL, Li X. The effects of glycogen synthase kinase-3beta in serotonin neurons. PLoS One. 2012;7(8):e43262. PMID: 22912839

  4. Tao J, Zhu M, Wang H, Afelik S, Vasievich MP, Chen XW, Zhu G, Jensen J, Ginsburg D, Zhang B. SEC23B is required for the maintenance of murine professional secretory tissues. Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):E2001-9 PMID: 22745161

  5. Goodnough LH, Chang AT, Treloar C, Yang J, Scacheri PC, Atit RP. Twist1 mediates repression of chondrogenesis by β-catenin to promote cranial bone progenitor specification. Development. 2012 139(23):4428-38. PMID: 23095887

  6. Bonfield TL, Hodges CA, Cotton CU, Drumm ML. Absence of the cystic fibrosis transmembrane regulator (Cftr) from myeloid-derived cells slows resolution of inflammation and infection. J Leukoc Biol. 2012 92(5):1111-22 PMID: 22859830

  7. Langhauser F, Göb E, Kraft P, Geis C, Schmitt J, Brede M, Göbel K, Helluy X, Pham M, Bendszus M, Jakob P, Stoll G, Meuth SG, Nieswandt B, McCrae KR, Kleinschnitz C. Kininogen deficiency protects from ischemic neurodegeneration in mice by reducing thrombosis, blood-brain-barrier damage and inflammation. Blood. 2012 PMID: 22936662

  8. Veenstra-VanderWeele J, Muller CL, Iwamoto H, Sauer JE, Owens WA, Shah CR, Cohen J, Mannangatti P, Jessen T, Thompson BJ, Ye R, Kerr TM, Carneiro AM, Crawley JN, Sanders-Bush E, McMahon DG, Ramamoorthy S, Daws LC, Sutcliffe JS, Blakely RD. Autism gene variant causes hyperserotonemia, serotonin receptor hypersensitivity, social impairment and repetitive behavior. Proc Natl Acad Sci U S A. 2012 109(14):5469-74. PMID: 22431635

  9. Sinha N, Pilder S, Vijayaraghavan S. Significant Expression Levels of Transgenic PPP1CC2 in Testis and Sperm Are Required to Overcome the Male Infertility Phenotype of Ppp1cc Null Mice. PLoS One. 2012;7(10):e47623 PMID: 23082183

  10. Wang W, Cui Y, Shen J, Jiang J, Chen S, Peng J, Wu Q. (2012) Salt-Sensitive Hypertension and Cardiac Hypertrophy in Transgenic Mice Expressing a Corin Variant Identified in Blacks. Hypertension. PMID: 22987923

  11. Ghosal K, Stathopoulos A, Thomas D, Phenis D, Vitek MP, Pimplikar SW. (2012) The Apolipoprotein-E-Mimetic COG112 Protects Amyloid Precursor Protein Intracellular Domain-Overexpressing Animals from Alzheimer's Disease-Like Pathological Features. Neurodegener Dis. PMID: 22965147

  12. Ketchart W, Smith KM, Krupka T, Wittmann BM, Hu Y, Rayman PA, Doughman YQ, Albert JM, Bai X, Finke JH, Xu Y, Exner AA, Montano MM. (2012) Inhibition of metastasis by HEXIM1 through effects on cell invasion and angiogenesis. Oncogene. PMID: 22964639

  13. Nelson VR, Heaney JD, Tesar PJ, Davidson NO, Nadeau JH. (2012) Transgenerational epigenetic effects of the Apobec1 cytidine deaminase deficiency on testicular germ cell tumor susceptibility and embryonic viability. Proc Natl Acad Sci U S A. 109(41):2766-73

  14. Seachrist DD, Johnson E, Magee C, Clay CM, Graham JK, Veeramachaneni DN, Keri RA. 2012 Overexpression of Follistatin in the Mouse Epididymis Disrupts Fluid Resorption and Sperm Transit in Testicular Excurrent Ducts. Biol Reprod

  15. Tian H, Lu X, Guo H, Corn D, Molter J, Wang B, Luo G, Lee Z 2012 Radio-deoxynucleoside Analogs used for Imaging tk Expression in a Transgenic Mouse Model of Induced Hepatocellular Carcinoma. Theranostics 2(6):597-606

  16. Kuwahara M, Yamashita M, Shinoda K, Tofukuji S, Onodera A, Shinnakasu R, Motohashi S, Hosokawa H, Tumes D, Iwamura C, Lefebvre V, Nakayama T. (2012) The transcription factor Sox4 is a downstream target of signaling by the cytokine TGF-β and suppresses T(H)2 differentiation. Nat Immunol. 13(8):778-86

  17. Niziolek PJ, Warman ML, Robling AG (2012) Mechanotransduction in bone tissue: the A214V and G171V mutations in Lrp5 enhance load-induced osteogenesis in a surface-selective manner. Bone

  18. Ishii A, Fyffe-Maricich SL, Furusho M, Miller RH, Bansal R (2012) ERK1/ERK2 MAPK Signaling is Required to Increase Myelin Thickness Independent of Oligodendrocyte Differentiation and Initiation of Myelination. J Neurosci. 32(26):8855-64.

  19. Pucilowska J, Puzerey PA, Karlo JC, Galán RF, Landreth GE. (2012) Disrupted ERK Signaling during Cortical Development Leads to Abnormal Progenitor Proliferation, Neuronal and Network Excitability and Behavior, Modeling Human Neuro-Cardio-Facial-Cutaneous and Related Syndromes. J Neurosci. 32(25):8663-77.

  20. Fox SR, Deneris ES. (2012) Engrailed is required in maturing serotonin neurons to regulate the cytoarchitecture and survival of the dorsal raphe nucleus. J Neurosci. 32(23):7832-42.

  21. Shim S, Kwan KY, Li M, Lefebvre V and Sestan N (2012) Cis-regulatory control of corticospinal system development and evolution. Nature 486:74-79
    • Sox4 and Sox11 conditional knockouts generated by the Lefebvre lab were used by Shim et al to show that Sox4 and Sox11 are required for specification and connectivity of the corticospinal tract in mice.

  22. Afelik S, Qu X, Hasrouni E, Bukys MA, Deering T, Nieuwoudt S, Rogers W, Macdonald RJ, Jensen J. (2012) Notch-mediated patterning and cell fate allocation of pancreatic progenitor cells. Development 139, 1744-1753 PMID: 22461559
    • The Jensen lab delineated the role of Notch signaling in the choice between pancreatic exocrine and endocrine fates with a doxycycline-regulated transgene expressing dominant negative Maml1 and nuclear EGFP.

  23. Garner AR, Rowland DC, Hwang SY, Baumgaertel K, Roth BL, Kentros C, and Mayford M (2012) Generation of a Synthetic Memory Trace. Science 335 (6075), 1513-1516.
    • Using a combination of genetic reagents including a transgenic mouse that the core generated for Bryan Roth's lab (Alexander et al., 2009 Neuron 63:27), Garner et al. generate synthetic memories in the brain of a mouse, illuminating the mechanisms of learning and memory.

  24. Heaney JD, Anderson EL, Michelson MV, Zechel JL, Conrad PA, Page DC, Nadeau JH. (2012) Germ cell pluripotency, premature differentiation and susceptibility to testicular teratomas in mice. Development 139(9):1577-86 PMID: 22438569

  25. Cui Y, Wang W, Dong N, Lou J, Srinivasan DK, Cheng W, Huang X, Liu M, Fang C, Peng J, Chen S, Wu S, Liu Z, Dong L, Zhou Y, Wu Q. (2012) Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy. Nature. 484(7393):246-50 PMID: 22437503
    • High blood pressure induced in pregnancy, pre-eclampsia, is a serious complication for mother and fetus, but is poorly understood. The Wu lab provides insight into pre-eclampsia by showing that atrial natriuretic peptide-converting enzyme (Corin) and its substrate atrial natriuretic peptide (ANP) are important in trophoblast invasion and spiral artery remodelling. In their absence, pregnant mice developed pre-ecamplsia.

  26. Jeyaraj D, Haldar SM, Wan X, McCauley MD, Ripperger JA, Hu K, Lu Y, Eapen BL, Sharma N, Ficker E, Cutler MJ, Gulick J, Sanbe A, Robbins J, Demolombe S, Kondratov RV, Shea SA, Albrecht U, Wehrens XH, Rosenbaum DS, Jain MK (2012) Circadian rhythms govern cardiac repolarization and arrhythmogenesis. Nature 483(7387):96-9
    • Heart attacks occur most frequently in the morning and evening. Using Klf15 transgenic and knockout mice, the Jain lab showed that the Klf15 transcription factor regulates circadian changes in heart rhythm, and that deficiency or excess of Klf15 increase susceptibility to arrhythmia.

  27. Dy P, Wang W, Bhattaram P, Wang Q, Wang L, Ballock RT, Lefebvre V (2012) Sox9 Directs Hypertrophic Maturation and Blocks Osteoblast Differentiation of Growth Plate Chondrocytes. Dev Cell 22:597-609
    • The Lefebvre laboratory showed that the Sox9 gene is required in the growth plate of bones for the proliferation and hypertrophy of chondrocytes, in addition to its known roles earlier in chondrogenesis.

  28. Espallergues J, Teegarden SL, Veerakumar A, Boulden J, Challis C, Jochems J, Chan M, Petersen T, Deneris E, Matthias P, Hahn CG, Lucki I, Beck SG, Berton O. (2012) HDAC6 regulates glucocorticoid receptor signaling in serotonin pathways with critical impact on stress resilience. J Neurosci. 32(13):4400-16.

  29. Mu L, Berti L, Masserdotti G, Covic M, Michaelidis TM, Doberauer K, Merz K, Rehfeld F, Haslinger A, Wegner M, Sock E, Lefebvre V, Couillard-Despres S, Aigner L, Berninger B, Lie DC.(2012) SoxC transcription factors are required for neuronal differentiation in adult hippocampal neurogenesis. J Neurosci 32(9):3067-3080

  30. Moriya K, Sakai K, Yan MH, Sakai T (2012) Fibronectin is essential for survival but is dispensable for proliferation of hepatocytes in acute liver injury. Hepatology

  31. Kyono A, Avishai N, Ouyang Z, Landreth GE, Murakami S. (2012) FGF and ERK signaling coordinately regulate mineralization-related genes and play essential roles in osteocyte differentiation. J Bone Miner Metab. 30:19-30

  32. Klokov D, Leskov K, Araki S, Zou Y, Goetz EM, Luo X, Willson D, Boothman DA. (2012) Low dose IR-induced IGF-1-sCLU expression: a p53-repressed expression cascade that interferes with TGFβ1 signaling to confer a pro-survival bystander effect. Oncogene. 2012