SOM     CCCC     CTSC
We are leaders in CRISPR/Cas9 gene targeting. We have completed over 100 CRISPR/Cas9 projects including knockouts, reporter and point mutant knockins and targeted transgenics. Our fees are low, our wait times are short and our success rates are high. New transgenic and targeted mice that we have created are featured in over 270 publications since 2004. We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center, the Clinical and Translational Science Collaborative, the Research Institute for Children's Health and by grants from the NIH, the Cystic Fibrosis Foundation and JobsOhio.

We are a component of the FIND Pipeline, which translates gene-directed approaches into therapies for inherited disease.
NameRoleOfficePhone
Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 623 368-1826


ctsc
Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.
Transgenics Targeting Chimeras Rederivation CRISPR/Cas Cryo IVF FET Other

Transgenic and Targeted Mice Made by the Core

2022
  1. Massey W, Osborn LJ, Banerjee R, Horak A, Fung KK, Orabi D, Chan ER, Sangwan N, Wang Z, Brown JM. Flavin-Containing Monooxygenase 3 (FMO3) Is Critical for Dioxin-Induced Reorganization of the Gut Microbiome and Host Insulin Sensitivity. Metabolites. 2022 18;12(4):364. PMID: 35448550

  2. Opoku E, Berisha S, Brubaker G, Robinet P, Smith JD. Oxidant resistant human apolipoprotein A-I functions similarly to the unmodified human isoform in delaying atherosclerosis progression and promoting atherosclerosis regression in hyperlipidemic mice. PLoS One. 2022 Feb 4;17(2):e0259751. PMID: 35120132

  3. LaForce GR, Farr JS, Liu J, Akesson C, Gumus E, Pinkard O, Miranda HC, Johnson K, Sweet TJ, Ji P, Lin A, Coller J, Philippidou P, Wagner EJ, Schaffer AE. Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. Neuron. 2022 PMID: 35139363

2021
  1. Gupta MK, Sahu A, Sun Y, Mohan ML, Kumar A, Zalavadia A, Wang X, Martelli EE, Stenson K, Witherow CP, Drazba J, Dasarathy S, Naga Prasad SV. Cardiac expression of microRNA-7 is associated with adverse cardiac remodeling. Sci Rep. 2021 11(1):22018. PMID: 34759299

  2. Wei W, Liu Z, Zhang C, Khoriaty R, Zhu M, Zhang B. A common human missense mutation of vesicle coat protein SEC23B leads to growth restriction and chronic pancreatitis in mice. J Biol Chem. 2021 Dec 23:101536. PMID: 34954140

  3. Vasu K, Ramachandiran I, Terenzi F, Khan D, China A, Khan K, Chechi A, Baleanu-Gogonea C, Gogonea V, Fox PL. The zinc-binding domain of mammalian prolyl-tRNA synthetase is indispensable for catalytic activity and organism viability. iScience. 2021 Feb 20;24(3):102215. PMID: 33748704

  4. Haseeb A, Kc R, Angelozzi M, de Charleroy C, Rux D, Tower RJ, Yao L, Pellegrino da Silva R, Pacifici M, Qin L, Lefebvre V. SOX9 keeps growth plates and articular cartilage healthy by inhibiting chondrocyte dedifferentiation/osteoblastic redifferentiation. Proc Natl Acad Sci U S A. 2021 118(8) PMID: 33597301

  5. Charrier A, Xu X, Guan BJ, Ngo J, Wynshaw-Boris A, Hatzoglou M, Buchner DA. Adipocyte-specific deletion of zinc finger protein 407 results in lipodystrophy and insulin resistance in mice. Mol Cell Endocrinol. 2021 PMID: 33285243

  6. Wu J, Venkata Subbaiah KC, Jiang F, Hedaya O, Mohan A, Yang T, Welle K, Ghaemmaghami S, Tang WHW, Small E, Yan C, Yao P. MicroRNA-574 regulates FAM210A expression and influences pathological cardiac remodeling. EMBO Mol Med. 2021 13(2):e12710. PMID: 33369227

  7. Xing G, Jing H, Yu Z, Chen P, Wang H, Xiong WC, Mei L. Membraneless condensates by Rapsn phase separation as a platform for neuromuscular junction formation. Neuron. 2021 PMID: 34033754

2020
  1. Billi AC, Ludwig JE, Fritz Y, Rozic R, Swindell WR, Tsoi LC, Gruzska D, Abdollahi-Roodsaz S, Xing X, Diaconu D, Uppala R, Camhi MI, Klenotic PA, Sarkar MK, Husni ME, Scher JU, McDonald C, Kahlenberg JM, Midura RJ, Gudjonsson JE, Ward NL. KLK6 expression in skin induces PAR1-mediated psoriasiform dermatitis and inflammatory joint disease. J Clin Invest. 2020 130(6):3151-3157. PMID: 32155135

  2. Tognatta R, Karl MT, Fyffe-Maricich SL, Popratiloff A, Garrison ED, Schenck JK, Abu-Rub M, Miller RH. Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity. Front Cell Neurosci. 2020 14:74. PMID: 32300294

  3. McHugh DR, Cotton CU, Hodges CA. Synergy between Readthrough and Nonsense Mediated Decay Inhibition in a Murine Model of Cystic Fibrosis Nonsense Mutations. Int J Mol Sci. 2020 22(1):E344. PMID: 33396210

  4. Thacker S, Sefyi M, Eng C. Alternative splicing landscape of the neural transcriptome in a cytoplasmic-predominant Pten expression murine model of autism-like Behavior. Transl Psychiatry. 2020 10(1):380. PMID: 33159038

  5. Jadhav VS, Lin PBC, Pennington T, Di Prisco GV, Jannu AJ, Xu G, Moutinho M, Zhang J, Atwood BK, Puntambekar SS, Bissel SJ, Oblak AL, Landreth GE, Lamb BT. Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice. Mol Neurodegener. 2020 15(1):62. PMID: 33115519

  6. Jaini R, Loya MG, King AT, Thacker S, Sarn NB, Yu Q, Stark GR, Eng C. Germline PTEN mutations are associated with a skewed peripheral immune repertoire in humans and mice. Hum Mol Genet. 2020 29(14):2353-2364. PMID: 32588888

  7. Cantsilieris S, Sunkin SM, Johnson ME, Anaclerio F, Huddleston J, Baker C, Dougherty ML, Underwood JG, Sulovari A, Hsieh P, Mao Y, Catacchio CR, Malig M, Welch AE, Sorensen M, Munson KM, Jiang W, Girirajan S, Ventura M, Lamb BT, Conlon RA, Eichler EE. An evolutionary driver of interspersed segmental duplications in primates. Genome Biol. 2020 21(1):202. PMID: 32778141

  8. Elitt MS, Barbar L, Shick HE, Powers BE, Maeno-Hikichi Y, Madhavan M, Allan KC, Nawash BS, Gevorgyan AS, Hung S, Nevin ZS, Olsen HE, Hitomi M, Schlatzer DM, Zhao HT, Swayze A, LePage DF, Jiang W, Conlon RA, Rigo F, Tesar PJ. Suppression of proteolipid protein rescues Pelizaeus-Merzbacher disease. Nature. 2020 PMID: 32610343

  9. Sparkenbaugh EM, Kasztan M, Henderson MW, Ellsworth P, Davis PR, Wilson KJ, Reeves B, Key NS, Strickland S, McCrae K, Pollock DM, Pawlinski R. High molecular weight kininogen contributes to early mortality and kidney dysfunction in a mouse model of sickle cell disease. J Thromb Haemost. 2020 PMID: 32573897

  10. Suehiro KI, Suto A, Suga K, Furuya H, Iwata A, Iwamoto T, Tanaka S, Kageyama T, Suzuki K, Hirose K, Lefebvre V, Nakajima H. Sox12 enhances Fbw7-mediated ubiquitination and degradation of GATA3 in Th2 cells. Cell Mol Immunol. 2020 PMID: 32152552

  11. Sarn N, Jaini R, Thacker S, Lee H, Dutta R, Eng C. Cytoplasmic-predominant Pten increases microglial activation and synaptic pruning in a murine model with autism-like phenotype. Mol Psychiatry. 2020 PMID: 320550089

2019
  1. Bruggeman LA, Wu Z, Luo L, Madhavan S, Drawz PE, Thomas DB, Barisoni L, O'Toole JF, Sedor JR. APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy. PLoS One. 2019 14(10):e0224408. PMID: 31661509

  2. Jay TR, von Saucken VE, Munoz B, Codocedo JF, Atwood BK, Lamb BT, Landreth GE. TREM2 is required for microglial instruction of astrocytic synaptic engulfment in neurodevelopment. Glia. 2019 67(10):1873-1892. PMID: 31265185

  3. Kang KS, Hong JM, Horan DJ, Lim KE, Bullock WA, Bruzzaniti A, Hann S, Warman ML, Robling AG. Induction of Lrp5 HBM-causing mutations in Cathepsin-K expressing cells alters bone metabolism. Bone. 2019 120:166-175. PMID: 304097579

  4. Kaupp S, Horan DJ, Lim KE, Feldman HA, Robling AG, Warman ML, Jacobsen CM. Combination therapy in the Col2a1G610C mouse model of Osteogenesis Imperfecta reveals an additive effect of enhancing LRP5 signaling and inhibiting TGFβ signaling on trabecular bone but not on cortical bone. Bone. 2019 PMID: 31648079

  5. Mark MD, Wollenweber P, Gesk A, Kösters K, Batzke K, Janoschka C, Maejima T, Han J, Deneris ES, Herlitze S. RGS2 drives male aggression in mice via the serotonergic system. Commun Biol. 2019 2:373. PMID: 31633064

  6. Miao Q, Hill MC, Chen F, Mo Q, Ku AT, Ramos C, Sock E, Lefebvre V, Nguyen H. SOX11 and SOX4 drive the reactivation of an embryonic gene program during murine wound repair. Nat Commun. 2019 10(1):4042. PMID: 31492871

  7. Donovan LJ, Spencer WC, Kitt MM, Eastman BA, Lobur KJ, Jiao K, Silver J, Deneris ES. Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife. 2019 PMID: 31355748

  8. Goiran T, Duplan E, Chami M, Bourgeois A, El Manaa W, Rouland L, Dunys J, Lauritzen I, You H, Stambolic V, Biféri MG, Barkats M, Pimplikar SW, Sergeant N, Colin M, Morais VA, Pardossi-Piquard R, Checler F, Alves da Costa C. β-Amyloid Precursor Protein Intracellular Domain Controls Mitochondrial Function by Modulating Phosphatase and Tensin Homolog-Induced Kinase 1 Transcription in Cells and in Alzheimer Mice Models. Biol Psychiatry. 2018 83(5):416-427. PMID: 28587718

  9. Shih DM, Zhu W, Schugar RC, Meng Y, Jia X, Miikeda A, Wang Z, Zieger M, Lee R, Graham M, Allayee H, Cantor RM, Mueller C, Brown JM, Hazen SL, Lusis AJ. Genetic Deficiency of Flavin-Containing Monooxygenase-3 ( Fmo3) Protects Against Thrombosis but Has Only a Minor Effect on Plasma Lipid Levels. Arterioscler Thromb Vasc Biol. 2019 PMID: 31070450

  10. Nandadasa S, Kraft CM, Wang LW, O'Donnell A, Patel R, Gee HY, Grobe K, Cox TC, Hildebrandt F, Apte SS. Secreted metalloproteases ADAMTS9 and ADAMTS20 have a non-canonical role in ciliary vesicle growth during ciliogenesis. Nat Commun. 2019 10(1):953. PMID: 30814516

  11. Shi Q, Ge Y, He W, Hu X, Yan R. RTN1 and RTN3 protein are differentially associated with senile plaques in Alzheimer's brains. Sci Rep. 2017 7(1):6145 PMID: 28733667

  12. Grandjean J, Corcoba A, Kahn MC, Upton AL, Deneris ES, Seifritz E, Helmchen F, Mann EO, Rudin M, Saab BJ. A brain-wide functional map of the serotonergic responses to acute stress and fluoxetine. Nat Commun. 2019 10(1):350. PMID: 30664643

  13. Lee H, Thacker S, Sarn N, Dutta R, Eng C. Constitutional mislocalization of Pten drives precocious maturation in oligodendrocytes and aberrant myelination in model of autism spectrum disorder. Transl Psychiatry. 2019 9(1):13. PMID: 30664625

  14. Baird RC, Li S, Wang H, Naga Prasad SV, Majdalany D, Perni U, Wu Q. Pregnancy-Associated Cardiac Hypertrophy in Corin-Deficient Mice: Observations in a Transgenic Model of Preeclampsia. Can J Cardiol. 2019 35(1):68-76. PMID: 30595185

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