SOM     CCCC     CTSC
We are leaders in CRISPR/Cas9 gene targeting. Our fees are low, and our success rates are high. New transgenic and targeted mice that we have created are featured in over 300 publications. We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center, the Clinical and Translational Science Collaborative, the Research Institute for Children's Health and by grants from the NIH, the Cystic Fibrosis Foundation and JobsOhio. We are a component of the FIND Pipeline, which translates gene-directed approaches into therapies for inherited disease.
NameRoleOfficePhone
Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 826 368-1826


ctsc
Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.

Transgenic and Targeted Mice Made by the Core

2024
  1. Sun Y, Chatterjee S, Lian X, Traylor Z, Sattiraju SR, Xiao Y, Dilliard SA, Sung Y-C, Kim M, Lee SM, Moore S, Wang X, Zhang D, Wu S, Basak P, Wang J, Liu J, Mann RJ, LePage DF, Jiang W, Abid S, Hennig M, Martinez A, Wustman BA, Lockhart DJ, Jain R, Conlon RA, Drumm, ML, Hodges CA, Siegwart DJ. In vivo editing of lung stem cells for durable gene correction in mice. (2024) Science 384,1196-1202

  2. Takita S, Jahan S, S Imanishi S, Harikrishnan H, LePage D, Mann RJ, Conlon RA, Miyagi M, Imanishi Y. Rhodopsin mislocalization drives ciliary dysregulation in a novel autosomal dominant retinitis pigmentosa knock-in mouse model. FASEB J. 2024 Apr 30;38(8):e23606. doi: 10.1096/fj.202302260RR. PMID: 38648465.

  3. Han X, Knauss EA, Fuente M, Li W, Conlon RA, LePage DF, Jiang W, Renna SA, McKenzie SE, Nieman MT. A Mouse Model of the Protease Activated Receptor 4 (PAR4) Pro310Leu Variant has Reduced Platelet Reactivity. J Thromb Haemost. 2024 Mar 18:S1538-7836(24)00161-2. PMID: 38508397.

  4. Vasudevan S, Senapati S, Pendergast M, Park PS. Aggregation of rhodopsin mutants in mouse models of autosomal dominant retinitis pigmentosa. Nat Commun. 2024 Feb 16;15(1):1451. PMID: 38365903; PMCID: PMC10873427.

2023
  1. Wei T, Sun Y, Cheng Q, Chatterjee S, Traylor Z, Johnson LT, Coquelin ML, Wang J, Torres MJ, Lian X, Wang X, Xiao Y, Hodges CA, Siegwart DJ. Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models. Nat Commun. 2023 Nov 11;14(1):7322. PMID: 37951948

  2. Esposito A, Klüppel M, Wilson BM, Meka SRK, Spagnoli A. CXCR4 mediates the effects of IGF-1R signaling in rodent bone homeostasis and fracture repair. Bone. 2023 166:116600 PMID: 36368465.

  3. Yang D, Wan X, Schwieterman N, Cavus O, Kacira E, Xu X, Laurita KR, Wold LE, Hund TJ, Mohler PJ, Deschênes I, Fu JD. MicroRNA-1 Deficiency Is a Primary Etiological Factor Disrupting Cardiac Contractility and Electrophysiological Homeostasis. Circ Arrhythm Electrophysiol. 2023 PMID: 38126205.

2022
  1. Li Y, Liu Z, Zhao Y, Yang J, Xiao TS, Conlon RA, Wang Z. PD-L1 expression is regulated by ATP-binding of the ERBB3 pseudokinase domain. Genes Dis. 2022 Dec 8;10(4):1702-1713. PMID: 37397533

  2. Kitt MM, Tabuchi N, Spencer WC, Robinson HL, Zhang XL, Eastman BA, Lobur KJ, Silver J, Mei L, Deneris ES. An adult-stage transcriptional program for survival of serotonergic connectivity. Cell Rep. 2022 Apr 19;39(3):110711 PMID: 35443166

  3. Zhang XL, Spencer WC, Tabuchi N, Kitt MM, Deneris ES. Reorganization of postmitotic neuronal chromatin accessibility for maturation of serotonergic identity. Elife. 2022 Apr 26;11:e75970. PMID: 35471146

  4. Massey W, Osborn LJ, Banerjee R, Horak A, Fung KK, Orabi D, Chan ER, Sangwan N, Wang Z, Brown JM. Flavin-Containing Monooxygenase 3 (FMO3) Is Critical for Dioxin-Induced Reorganization of the Gut Microbiome and Host Insulin Sensitivity. Metabolites. 2022 18;12(4):364. PMID: 35448550

  5. Opoku E, Berisha S, Brubaker G, Robinet P, Smith JD. Oxidant resistant human apolipoprotein A-I functions similarly to the unmodified human isoform in delaying atherosclerosis progression and promoting atherosclerosis regression in hyperlipidemic mice. PLoS One. 2022 Feb 4;17(2):e0259751. PMID: 35120132

  6. LaForce GR, Farr JS, Liu J, Akesson C, Gumus E, Pinkard O, Miranda HC, Johnson K, Sweet TJ, Ji P, Lin A, Coller J, Philippidou P, Wagner EJ, Schaffer AE. Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. Neuron. 2022 PMID: 35139363

  7. Bohne P, Volkmann A, Schwarz MK, Mark MD. Deletion of the P/Q-type calcium channel from serotonergic neurons drives male aggression in mice. J Neurosci. 2022 Jul 18;42(34):6637–53. PMID: 35853721

2021
  1. Gupta MK, Sahu A, Sun Y, Mohan ML, Kumar A, Zalavadia A, Wang X, Martelli EE, Stenson K, Witherow CP, Drazba J, Dasarathy S, Naga Prasad SV. Cardiac expression of microRNA-7 is associated with adverse cardiac remodeling. Sci Rep. 2021 11(1):22018. PMID: 34759299

  2. Wei W, Liu Z, Zhang C, Khoriaty R, Zhu M, Zhang B. A common human missense mutation of vesicle coat protein SEC23B leads to growth restriction and chronic pancreatitis in mice. J Biol Chem. 2021 Dec 23:101536. PMID: 34954140

  3. Vasu K, Ramachandiran I, Terenzi F, Khan D, China A, Khan K, Chechi A, Baleanu-Gogonea C, Gogonea V, Fox PL. The zinc-binding domain of mammalian prolyl-tRNA synthetase is indispensable for catalytic activity and organism viability. iScience. 2021 Feb 20;24(3):102215. PMID: 33748704

  4. Haseeb A, Kc R, Angelozzi M, de Charleroy C, Rux D, Tower RJ, Yao L, Pellegrino da Silva R, Pacifici M, Qin L, Lefebvre V. SOX9 keeps growth plates and articular cartilage healthy by inhibiting chondrocyte dedifferentiation/osteoblastic redifferentiation. Proc Natl Acad Sci U S A. 2021 118(8) PMID: 33597301

  5. Charrier A, Xu X, Guan BJ, Ngo J, Wynshaw-Boris A, Hatzoglou M, Buchner DA. Adipocyte-specific deletion of zinc finger protein 407 results in lipodystrophy and insulin resistance in mice. Mol Cell Endocrinol. 2021 PMID: 33285243

  6. Wu J, Venkata Subbaiah KC, Jiang F, Hedaya O, Mohan A, Yang T, Welle K, Ghaemmaghami S, Tang WHW, Small E, Yan C, Yao P. MicroRNA-574 regulates FAM210A expression and influences pathological cardiac remodeling. EMBO Mol Med. 2021 13(2):e12710. PMID: 33369227

  7. Xing G, Jing H, Yu Z, Chen P, Wang H, Xiong WC, Mei L. Membraneless condensates by Rapsn phase separation as a platform for neuromuscular junction formation. Neuron. 2021 PMID: 34033754

  8. Han Y, Xia G, Srisai D, Meng F, He Y, Ran Y, He Y, Farias M, Hoang G, Tóth I, Dietrich MO, Chen MH, Xu Y, Wu Q. Deciphering an AgRP-serotoninergic neural circuit in distinct control of energy metabolism from feeding. Nat Commun. 2021 Jun 10;12(1):3525. PMID: 34112797

2020
  1. Billi AC, Ludwig JE, Fritz Y, Rozic R, Swindell WR, Tsoi LC, Gruzska D, Abdollahi-Roodsaz S, Xing X, Diaconu D, Uppala R, Camhi MI, Klenotic PA, Sarkar MK, Husni ME, Scher JU, McDonald C, Kahlenberg JM, Midura RJ, Gudjonsson JE, Ward NL. KLK6 expression in skin induces PAR1-mediated psoriasiform dermatitis and inflammatory joint disease. J Clin Invest. 2020 130(6):3151-3157. PMID: 32155135

  2. Tognatta R, Karl MT, Fyffe-Maricich SL, Popratiloff A, Garrison ED, Schenck JK, Abu-Rub M, Miller RH. Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity. Front Cell Neurosci. 2020 14:74. PMID: 32300294

  3. McHugh DR, Cotton CU, Hodges CA. Synergy between Readthrough and Nonsense Mediated Decay Inhibition in a Murine Model of Cystic Fibrosis Nonsense Mutations. Int J Mol Sci. 2020 22(1):E344. PMID: 33396210

  4. Thacker S, Sefyi M, Eng C. Alternative splicing landscape of the neural transcriptome in a cytoplasmic-predominant Pten expression murine model of autism-like Behavior. Transl Psychiatry. 2020 10(1):380. PMID: 33159038

  5. Jadhav VS, Lin PBC, Pennington T, Di Prisco GV, Jannu AJ, Xu G, Moutinho M, Zhang J, Atwood BK, Puntambekar SS, Bissel SJ, Oblak AL, Landreth GE, Lamb BT. Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice. Mol Neurodegener. 2020 15(1):62. PMID: 33115519

  6. Jaini R, Loya MG, King AT, Thacker S, Sarn NB, Yu Q, Stark GR, Eng C. Germline PTEN mutations are associated with a skewed peripheral immune repertoire in humans and mice. Hum Mol Genet. 2020 29(14):2353-2364. PMID: 32588888

  7. Cantsilieris S, Sunkin SM, Johnson ME, Anaclerio F, Huddleston J, Baker C, Dougherty ML, Underwood JG, Sulovari A, Hsieh P, Mao Y, Catacchio CR, Malig M, Welch AE, Sorensen M, Munson KM, Jiang W, Girirajan S, Ventura M, Lamb BT, Conlon RA, Eichler EE. An evolutionary driver of interspersed segmental duplications in primates. Genome Biol. 2020 21(1):202. PMID: 32778141

  8. Elitt MS, Barbar L, Shick HE, Powers BE, Maeno-Hikichi Y, Madhavan M, Allan KC, Nawash BS, Gevorgyan AS, Hung S, Nevin ZS, Olsen HE, Hitomi M, Schlatzer DM, Zhao HT, Swayze A, LePage DF, Jiang W, Conlon RA, Rigo F, Tesar PJ. Suppression of proteolipid protein rescues Pelizaeus-Merzbacher disease. Nature. 2020 PMID: 32610343

  9. Sparkenbaugh EM, Kasztan M, Henderson MW, Ellsworth P, Davis PR, Wilson KJ, Reeves B, Key NS, Strickland S, McCrae K, Pollock DM, Pawlinski R. High molecular weight kininogen contributes to early mortality and kidney dysfunction in a mouse model of sickle cell disease. J Thromb Haemost. 2020 PMID: 32573897

  10. Suehiro KI, Suto A, Suga K, Furuya H, Iwata A, Iwamoto T, Tanaka S, Kageyama T, Suzuki K, Hirose K, Lefebvre V, Nakajima H. Sox12 enhances Fbw7-mediated ubiquitination and degradation of GATA3 in Th2 cells. Cell Mol Immunol. 2020 PMID: 32152552

  11. Sarn N, Jaini R, Thacker S, Lee H, Dutta R, Eng C. Cytoplasmic-predominant Pten increases microglial activation and synaptic pruning in a murine model with autism-like phenotype. Mol Psychiatry. 2020 PMID: 320550089

  12. Azimi Z, Barzan R, Spoida K, Surdin T, Wollenweber P, Mark MD, Herlitze S, Jancke D. Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input. Elife. 2020 Apr 7;9:e53552. PMID: 32252889

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