SOM     CCCC     CTSC
We are leaders in CRISPR/Cas9 gene targeting. We have completed over 70 CRISPR/Cas9 projects including knockouts, reporter and point mutant knockins and targeted transgenics. Our fees are low, our wait times are short and our success rates are high. New transgenic and targeted mice that we have created are featured in over 270 publications since 2004. We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center, the Clinical and Translational Science Collaborative and by grants from the Cystic Fibrosis Foundation.

NameRoleOfficePhone
Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 623 368-1826


ctsc
Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.
Transgenics Targeting Chimeras Rederivation CRISPR/Cas Cryo IVF FET Other

Transgenic and Targeted Mice Made by the Core

2020
  1. McHugh DR, Cotton CU, Hodges CA. Synergy between Readthrough and Nonsense Mediated Decay Inhibition in a Murine Model of Cystic Fibrosis Nonsense Mutations. Int J Mol Sci. 2020 22(1):E344. PMID: 33396210

  2. Thacker S, Sefyi M, Eng C. Alternative splicing landscape of the neural transcriptome in a cytoplasmic-predominant Pten expression murine model of autism-like Behavior. Transl Psychiatry. 2020 10(1):380. PMID: 33159038

  3. Jadhav VS, Lin PBC, Pennington T, Di Prisco GV, Jannu AJ, Xu G, Moutinho M, Zhang J, Atwood BK, Puntambekar SS, Bissel SJ, Oblak AL, Landreth GE, Lamb BT. Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice. Mol Neurodegener. 2020 15(1):62. PMID: 33115519

  4. Jaini R, Loya MG, King AT, Thacker S, Sarn NB, Yu Q, Stark GR, Eng C. Germline PTEN mutations are associated with a skewed peripheral immune repertoire in humans and mice. Hum Mol Genet. 2020 29(14):2353-2364. PMID: 32588888

  5. Cantsilieris S, Sunkin SM, Johnson ME, Anaclerio F, Huddleston J, Baker C, Dougherty ML, Underwood JG, Sulovari A, Hsieh P, Mao Y, Catacchio CR, Malig M, Welch AE, Sorensen M, Munson KM, Jiang W, Girirajan S, Ventura M, Lamb BT, Conlon RA, Eichler EE. An evolutionary driver of interspersed segmental duplications in primates. Genome Biol. 2020 21(1):202. PMID: 32778141

  6. Elitt MS, Barbar L, Shick HE, Powers BE, Maeno-Hikichi Y, Madhavan M, Allan KC, Nawash BS, Gevorgyan AS, Hung S, Nevin ZS, Olsen HE, Hitomi M, Schlatzer DM, Zhao HT, Swayze A, LePage DF, Jiang W, Conlon RA, Rigo F, Tesar PJ. Suppression of proteolipid protein rescues Pelizaeus-Merzbacher disease. Nature. 2020 PMID: 32610343

  7. Sparkenbaugh EM, Kasztan M, Henderson MW, Ellsworth P, Davis PR, Wilson KJ, Reeves B, Key NS, Strickland S, McCrae K, Pollock DM, Pawlinski R. High molecular weight kininogen contributes to early mortality and kidney dysfunction in a mouse model of sickle cell disease. J Thromb Haemost. 2020 PMID: 32573897

  8. Suehiro KI, Suto A, Suga K, Furuya H, Iwata A, Iwamoto T, Tanaka S, Kageyama T, Suzuki K, Hirose K, Lefebvre V, Nakajima H. Sox12 enhances Fbw7-mediated ubiquitination and degradation of GATA3 in Th2 cells. Cell Mol Immunol. 2020 PMID: 32152552

  9. Sarn N, Jaini R, Thacker S, Lee H, Dutta R, Eng C. Cytoplasmic-predominant Pten increases microglial activation and synaptic pruning in a murine model with autism-like phenotype. Mol Psychiatry. 2020 PMID: 320550089

2019
  1. Jay TR, von Saucken VE, Munoz B, Codocedo JF, Atwood BK, Lamb BT, Landreth GE. TREM2 is required for microglial instruction of astrocytic synaptic engulfment in neurodevelopment. Glia. 2019 67(10):1873-1892. PMID: 31265185

  2. Kang KS, Hong JM, Horan DJ, Lim KE, Bullock WA, Bruzzaniti A, Hann S, Warman ML, Robling AG. Induction of Lrp5 HBM-causing mutations in Cathepsin-K expressing cells alters bone metabolism. Bone. 2019 120:166-175. PMID: 304097579

  3. Kaupp S, Horan DJ, Lim KE, Feldman HA, Robling AG, Warman ML, Jacobsen CM. Combination therapy in the Col2a1G610C mouse model of Osteogenesis Imperfecta reveals an additive effect of enhancing LRP5 signaling and inhibiting TGFβ signaling on trabecular bone but not on cortical bone. Bone. 2019 PMID: 31648079

  4. Mark MD, Wollenweber P, Gesk A, Kösters K, Batzke K, Janoschka C, Maejima T, Han J, Deneris ES, Herlitze S. RGS2 drives male aggression in mice via the serotonergic system. Commun Biol. 2019 2:373. PMID: 31633064

  5. Miao Q, Hill MC, Chen F, Mo Q, Ku AT, Ramos C, Sock E, Lefebvre V, Nguyen H. SOX11 and SOX4 drive the reactivation of an embryonic gene program during murine wound repair. Nat Commun. 2019 10(1):4042. PMID: 31492871

  6. Donovan LJ, Spencer WC, Kitt MM, Eastman BA, Lobur KJ, Jiao K, Silver J, Deneris ES. Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife. 2019 PMID: 31355748

  7. Goiran T, Duplan E, Chami M, Bourgeois A, El Manaa W, Rouland L, Dunys J, Lauritzen I, You H, Stambolic V, Biféri MG, Barkats M, Pimplikar SW, Sergeant N, Colin M, Morais VA, Pardossi-Piquard R, Checler F, Alves da Costa C. β-Amyloid Precursor Protein Intracellular Domain Controls Mitochondrial Function by Modulating Phosphatase and Tensin Homolog-Induced Kinase 1 Transcription in Cells and in Alzheimer Mice Models. Biol Psychiatry. 2018 83(5):416-427. PMID: 28587718

  8. Shih DM, Zhu W, Schugar RC, Meng Y, Jia X, Miikeda A, Wang Z, Zieger M, Lee R, Graham M, Allayee H, Cantor RM, Mueller C, Brown JM, Hazen SL, Lusis AJ. Genetic Deficiency of Flavin-Containing Monooxygenase-3 ( Fmo3) Protects Against Thrombosis but Has Only a Minor Effect on Plasma Lipid Levels. Arterioscler Thromb Vasc Biol. 2019 PMID: 31070450

  9. Nandadasa S, Kraft CM, Wang LW, O'Donnell A, Patel R, Gee HY, Grobe K, Cox TC, Hildebrandt F, Apte SS. Secreted metalloproteases ADAMTS9 and ADAMTS20 have a non-canonical role in ciliary vesicle growth during ciliogenesis. Nat Commun. 2019 10(1):953. PMID: 30814516

  10. Shi Q, Ge Y, He W, Hu X, Yan R. RTN1 and RTN3 protein are differentially associated with senile plaques in Alzheimer's brains. Sci Rep. 2017 7(1):6145 PMID: 28733667

  11. Grandjean J, Corcoba A, Kahn MC, Upton AL, Deneris ES, Seifritz E, Helmchen F, Mann EO, Rudin M, Saab BJ. A brain-wide functional map of the serotonergic responses to acute stress and fluoxetine. Nat Commun. 2019 10(1):350. PMID: 30664643

  12. Lee H, Thacker S, Sarn N, Dutta R, Eng C. Constitutional mislocalization of Pten drives precocious maturation in oligodendrocytes and aberrant myelination in model of autism spectrum disorder. Transl Psychiatry. 2019 9(1):13. PMID: 30664625

  13. Baird RC, Li S, Wang H, Naga Prasad SV, Majdalany D, Perni U, Wu Q. Pregnancy-Associated Cardiac Hypertrophy in Corin-Deficient Mice: Observations in a Transgenic Model of Preeclampsia. Can J Cardiol. 2019 35(1):68-76. PMID: 30595185

2018
  1. Dudiki T, Joudeh N, Sinha N, Goswami S, Eisa A, Kline D, Vijayaraghavan S. The protein phosphatase isoform PP1γ1 substitutes for PP1γ2 to support spermatogenesis but not normal sperm function and fertility. Biol Reprod. 2019 100(3):721-736. PMID: 30379985

  2. Tanaka S, Suto A, Iwamoto T, Kageyama T, Tamachi T, Takatori H, Suzuki K, Hirose K, Ohara O, Lefebvre V, Nakajima H. Sox12 promotes T reg differentiation in the periphery during colitis. J Exp Med. 2018 Sep 6. PMID: 30190287

  3. Xiang X, Piers TM, Wefers B, Zhu K, Mallach A, Brunner B, Kleinberger G, Song W, Colonna M, Herms J, Wurst W, Pocock JM, Haass C. Mol Neurodegener. The Trem2 R47H Alzheimer's risk variant impairs splicing and reduces Trem2 mRNA and protein in mice but not in humans. 2018 13(1):49. PMID: 30185230

  4. Liu CF, Angelozzi M, Haseeb A, Lefebvre V. SOX9 is dispensable for the initiation of epigenetic remodeling and the activation of marker genes at the onset of chondrogenesis. Development. 2018 145(14). PMID: 30021842

  5. Zhu W, Buffa JA, Wang Z, Warrier M, Schugar R, Shih DM, Gupta N, Gregory JC, Org E, Fu X, Li L, DiDonato JA, Lusis AJ, Brown JM, Hazen SL. Flavin monooxygenase 3, the host hepatic enzyme in the metaorganismal trimethylamine N-oxide-generating pathway, modulates platelet responsiveness and thrombosis risk. J Thromb Haemost. 2018 PMID: 29981269

  6. McHugh DR, Steele MS, Valerio DM, Miron A, Mann RJ, LePage DF, Conlon RA, Cotton CU, Drumm ML, Hodges CA. A G542X cystic fibrosis mouse model for examining nonsense mutation directed therapies. PLoS One. 2018 13(6) PMID: 29924856
    • A disease-variant allele made by CRISPR/Cas9 mutagenesis.

  7. Cheng-Hathaway PJ, Reed-Geaghan EG, Jay TR, Casali BT, Bemiller SM, Puntambekar SS, von Saucken VE, Williams RY, Karlo JC, Moutinho M, Xu G, Ransohoff RM, Lamb BT, Landreth GE. The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease. Mol Neurodegener. 2018 13(1):29 PMID: 29859094
    • A disease-variant allele made by CRISPR/Cas9 mutagenesis.

  8. Meier ID, Walker MP, Matera AG. Gemin4 is an essential gene in mice, and its overexpression in human cells causes relocalization of the SMN complex to the nucleoplasm. Biol Open. 2018 PMID: 29371219

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