We create transgenic mice, knockout mice (null mutations, knockins, targeted point mutations, humanized genes, conditional knockouts) and chimeric mice, provide rederivation, cryopreservation services and establish embryonic stem cell lines for the Cleveland Biomedical Research Community.

     Services are contracted on a fee-for-service, first-come-first-serve basis. Our rates are low, our wait times are short, and we have an oustanding record of success with many publications resulting from our work.

     We are supported by user fees, the Case Western Reserve University School of Medicine and the Case Comprehensive Cancer Center.

• Transgenics
• Targeting
• Chimeras
• Rederivation
• Cryopreservation
• Generation of new ES cell lines
• ES cell transfection
• ES cell homozygosis
• In vitro Fertilization (IVF)
• Intracytoplasmic Sperm Injection (ICSI)
• Frozen Embryo Transfer (FET)
• Ovariectomy, Vasectomy and Other Surgeries

• Cost of Services
• Wait Times
• CTSC $10,000 Pilot Project Grants
• NIH Supplements for genetrap cell lines
• Mice: The Basics
• How to Breed Mice
• How to Design Gene Targeting Vectors
• Strategies: Transgenic or Gene Targeted Mouse?
• Existing Mutants and Where to Get Them
• Commercial DNA Cloning and Genotyping Services
• What an Incoming Investigator Needs to Know
• Health status of Tg Facility mice
• Protocols
• Resources and Links
• Publications resulting from our work

     The Case Transgenic and Targeting Facility office is located in Wolstein Research Bldg 2521.

Left to Right: Ron, Rachel, David, Weihong